Potential chemopreventive activities of a polyphenol rich purified extract from olive mill wastewater on colon cancer cells


Olive oil, a major feature of the Mediterranean diet, is an abundant source of phenolic com- pounds. Olive oil production is associated with the generation of waste material, termed ‘olive mill wastewater’ (OMWW), that has been reported to be enriched in soluble polyphe- nols. Given the known beneficial activity of polyphenols, we investigated whether the use of purified extracts from OMWW, termed A009, rich in hydroxytyrosol, might have antican- cer activities on colon cancer (CC) cell lines in vitro and in vivo and could represent a chemopreventive preparation for CC. A009 from different batches inhibited proliferation, migration, invasion, adhesion, sprouting of CC cells and release of angiogenic, pro- inflammatory cytokines (VEGF, IL-8). Our data demonstrate that a novel purified, polyphenol enriched extract, obtained from food industry waste material, with similar activity than pu- rified hydroxytyrosol but easier to produce in large quantities and with an environment- sensitive approach, has potential cancer chemopreventive properties for colon cancer cells.